Fig. 2
FTO inhibits PTC cell proliferation in vitro and in vivo. a Relative protein expression of FTO in the normal thyroid follicular epithelial cells and PTC cells as determined by western blotting. b Knockdown efficiency of si-FTO-1/2 in K1 cells as determined by qRT-PCR and western blotting. c Overexpression efficiency of FTO and FTO-mut in TPC1 cells as determined by qRT-PCR and western blotting. d Total m6A modification levels after FTO knockdown or overexpression in PTC cells as determined by m6A RNA Methylation Quantification Kit. e-f CCK-8 assay (e) and colony formation assay (f) showing proliferation ability in K1 cells after FTO knockdown. g-h CCK-8 assay (g) and colony formation assay (h) showing proliferation ability after FTO overexpression in TPC1 cells. i Representative image of subcutaneous tumors excised from nude mice model administered with K1 cells infected with sh-NC and sh-FTO (n = 5). j-k Tumor volumes (j) and tumor weights (k) were determined after excision from nude mice in the above-mentioned groups. l Representative image of subcutaneous tumors excised from nude mice model administered with TPC1 cells infected with empty vector, FTO and FTO-mut. m-n Tumor volumes (m) and tumor weights (n) were determined after excision from nude mice model in the above-mentioned groups. *P < 0.05, **P < 0.01