Fig. 2

NFIL3 promotes the proliferation and metastasis of TNBC cells in vitro and in vivo. A. Overexpression of NFIL3 promoted the proliferation of Hs578T cells, as determined by the cell counting test (*p < 0.05). B. Overexpression of NFIL3 promoted the clone formation rate of Hs578T cells (*p < 0.05). C. Knockdown of NFIL3 inhibited the proliferation of Hs578T cells, as determined by the cell counting test (*p < 0.05). D. Knockdown of NFIL3 inhibits the clone formation rate of Hs578T cells (**p < 0.01). E. NFIL3 overexpression promoted the growth of Hs578T cells in a xenograft mouse model, as determined by evaluating the weight and volume of tumors (*p < 0.05, **p < 0.01). F. Knockdown of NFIL3 inhibited the growth of Hs578T cells in a xenograft mouse model by evaluating the weight and volume of tumors (*p < 0.05). G. Overexpression of NFIL3 promotes the wound healing of Hs578T cells (*p < 0.05, **p < 0.01). H. Overexpression of NFIL3 enhances the migration of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). I. Overexpression of NFIL3 enhances the invasion of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). J. Knockdown of NFIL3 inhibits the wound healing of Hs578T cells (*p < 0.05, **p < 0.01). K. Knockdown of NFIL3 inhibited the migration of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). L. Knockdown of NFIL3 inhibited the invasion of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). M. Overexpression of NFIL3 promotes lung metastasis of Hs578T cells using a tail vein injection model (magnification, 100×; scale bars = 200 μm; the black arrow indicates the metastatic foci; ***p < 0.001). N. Knockdown of NFIL3 suppresses lung metastasis of Hs578T cells using a tail vein injection model (magnification, 100×; scale bars = 200 μm; the black arrow indicates the metastatic foci; *p < 0.05)