Fig. 6

NFKBIA blocks the proliferation and metastasis of TNBC cells. A. Overexpression of NFKBIA inhibited the proliferation of Hs578T cells, as determined by the cell counting test (*p < 0.05). B. Overexpression of NFKBIA inhibits the clone formation rate of Hs578T cells (*p < 0.05). C. Knockdown of NFKBIA promoted the proliferation of Hs578T cells, as determined by the cell counting test (*p < 0.05). D. Knockdown of NFKBIA promotes the clone formation rate of Hs578T cells (**p < 0.01). E. NFKBIA overexpression inhibited the growth of Hs578T cells in a xenograft mouse model by evaluating the weight and volume of tumors (**p < 0.01). F. Knockdown of NFKBIA promotes the growth of Hs578T cells in a xenograft mouse model by evaluating the weight and volume of tumors (**p < 0.01). G. Overexpression of NFKBIA inhibits the wound healing of Hs578T cells (*p < 0.05, **p < 0.01). H. Overexpression of NFKBIA inhibited the migration of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). I. Overexpression of NFKBIA inhibited the invasion of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). J. Overexpression of NFKBIA inhibited lung metastasis of Hs578T cells using a tail vein injection model (magnification, 100×; scale bars = 200 μm; the black arrow indicates the metastatic foci; *p < 0.05). K. Knockdown of NFKBIA promotes the wound healing of Hs578T cells (*p < 0.05, ***p < 0.001). L. Knockdown of NFKBIA promoted the migration of Hs578T cells, as detected by Transwell assay (magnification, 200×; *p < 0.05, ***p < 0.001). M. Knockdown of NFKBIA promoted the invasion of Hs578T cells, as detected by Transwell assay (magnification, 200×; ***p < 0.001). N. Knockdown of NFKBIA promotes lung metastasis of Hs578T cells using a tail vein injection model (magnification, 100×; scale bars = 200 μm; the black arrow indicates the metastatic foci; *p < 0.05)