Fig. 8
From: Increased Drp1 promotes autophagy and ESCC progression by mtDNA stress mediated cGAS-STING pathway
Blocking cGAS-STING pathway inhibits mtDNA stress induced ESCC progression. (A) Cell proliferation was evaluated by EdU incorporation assay in KYSE-30 cells treatment with indication. Scale bar: 200 μm. (B) Colony-forming potential were detected in KYSE-30 cells treatment with indication. Scale bar: 10 mm. (C and D) Tumor growth curves and weight of subcutaneous xenograft tumor model developed from ESCC cells treatment with indication (n = 5). (E) Representative IHC staining images of Ki67 and LC3B in xenograft tumors developed from KYSE-30 cells treatment with indication. Scale bar: 100 μm. The data shown are the mean ± SEM from three separate experiments. Drp1, expression vector encoding Drp1; EV, empty vector; NC, negative control; siSTING, siRNAs against STING; sicGAS, siRNAs against cGAS; CQ, chloroquine. P value from t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001