Fig. 6

Proteome Profiler analysis of CTCDOs/CTCDOs-derived xenografts versus XDOs/XDOs-derived xenografts and selected protein validations on CRC CTCs. A Hierarchical clustering of Proteome Profiler results obtained on CTCDOs/XDOs and CTCDOs-derived tumor xenografts /XDOs-derived tumor xenografts (n = 2 pools of 3 tumors each). Clusters, identified for either antibodies or samples and based on optimal cut of dendrograms, are indicated by colored bars adjacent to dendrograms. The values represented by the heatmap correspond to normalized intensities of antibodies, standardized over the sample set analyzed (z score). A list of Proteome Profiler antibodies is reported in Additional file 4: Table S3. B Immunofluorescence analysis of stemness-associated factors Goosecoid and PDX1 on CTCs isolated from the peripheral blood of two CRC patients. C Immunofluorescence analysis of stress-associated factors HIF-1α and phospho-HSP27 on CTCs isolated from the peripheral blood of two CRC patients. Images are representative of at least three CTCs isolated from three different patients. Magnification 60x, 5x zoom, bar 10 μM