Fig. 4

HDAC7 deacetylated β-catenin at Lys49 and promoted its translocation into the nucleus to activate FGF18 expression. a HDAC7 and β-catenin interacted in NSCLC cells. b Two-color immunofluorescence analysis showing significant colocalization of HDAC7 with β-catenin in A549 and SK-LU-1 cells. DAPI (blue), HDAC7 (green), and β-catenin (red). c Representative western blotting results showing HDAC7 deacetylated β-catenin at Lys49 and phosphorylated β-catenin at Ser45. d Representative western blotting showing upregulated HDAC7 facilitated β-catenin nuclear translocation. e Representative western blotting of HDAC7, FGF18, β-catenin, and TCF4 in NSCLC cells after LF3 treatment. β-actin was used as an internal control to normalize the total protein amount. GAPDH and Lamin B1 were used as the loading control to cytosolic protein and nuclear protein, respectively. DAPI fluorescence marking the cell nucleus. LV, lentivirus. IP, immunoprecipitation. IB, immunoblot