Fig. 3
From: Partial p53 reactivation is sufficient to induce cancer regression
E177R activation induces regression of p53-deficient AML in vivo. a-b 106 AML cells of indicated genotype were transplanted into sublethally irradiated 129X1 albino x B6 albino (F1 hybrid) mice. After engraftment was confirmed by bioluminescence imaging (BLI), mice were treated 1 week with daily i.p. injections of either vehicle (corn oil) or 1 mg tamoxifen. Mice were euthanized when humane endpoint criteria were reached. a BLI of representative mice. b Kaplan-Meier survival graph. Log-rank (Mantel-Cox) test for LSL mock vs. Tam. c-d 106 LSL-Cas9 AML cells transduced ex vivo with lentivirus encoding either Trp53-directed sgRNA (sg-p53) or scrambled control sgRNA (sg-scr) were transplanted into mice as in a and treated 1 week with daily i.p. injections of 1 mg tamoxifen. Mice were euthanized when humane endpoint criteria were reached. c BLI of both cohorts. d Kaplan-Meier survival graph. Log-rank (Mantel-Cox) test. e Immunohistochemistry for p53 of relapsed leukemia samples from both cohorts