Fig. 3

BD enhances the anti-tumor activity of GEM in KPC mouse model. a The timeline of experiments on KPC model. b Body weight of the mice was measured every other day. c-d Representative macroscopic images and tumor weight of PDAC in KPC mice treated with BD, GEM and their combination. e–f Histopathological analysis of the tumors of KPC mice and representative images of Masson’s trichrome staining. g Quantification of Masson’s trichrome staining in the tumor tissue of KPC mice. h Serum levels of AST, ALT and creatinine of KPC mice (n = 4 to 7 per group). i Paraffin-embedded KPC tumor tissues were stained with IHC for evaluation of Ki-67 and Nrf2 expression (Scale bar: 50 μm). Data were presented as the mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the control group; n.s., no significant. ^#p < 0.05 and ^##p < 0.01 compared with the GEM alone treatment group