Fig. 1

DICER1-AS1 is downregulated in pancreatic cancer tissues and negatively correlated with glycolysis pathway. A Venn diagram (left panel) indicating the identification of differentially expressed lncRNAs (|log2FoldChange|> 1, P < 0.05) in the TCGA database. The right panel showing the identification of glycolysis-related lncRNAs by GSEA analyses. B Bubble plot showing the GSEA analyses of 7 lncRNAs using a glycolysis-related gene set in the TCGA database. NES, normalized enrichment score. C Boxplot showing the relative level of DICER1-AS1 between normal and tumor tissues in pancreatic cancer (GSE41368). D GSEA enrichment plot of DICER1-AS1 using glycolysis-related gene set in the TCGA database. NES, normalized enrichment score. E The relative transcript levels of DICER1-AS1 in PC tissues with different status of tumor stage and progression. F Kaplan–Meier analyses of overall survival (OS) (cutoff value = 193.92), disease-free interval (DFI) (cutoff value = 190.87), progression-free interval (PFI) (cutoff value = 177.20), and disease-specific survival (DSS) (cutoff value = 177.20) in pancreatic cancer patients with low and high levels of DICER1-AS1 using the log-rank test. G Representative FISH images of DICER1-AS1 expression in adjacent noncancerous pancreatic (NP) tissues and pancreatic cancer (PC) tissues. Blue, DAPI staining; Red, DICER1-AS1 staining; Scale bar = 20 µm. H Real-time qRT-PCR showing the relative DICER1-AS1 levels in a normal pancreatic cell line (HPDE) and PC cell lines (SW1990, AsPC-1, PANC-1, BxPC-3). I Real-time qRT-PCR showing in the subcellular fractions of DICER1-AS1 (left panel) in BxPC-3 and PANC-1 cells (mean ± SD, n = 3). The PCR products were run on a 2% agarose gel and U6 and β-actin were used as nuclear and cytoplasmic markers, respectively (right panel). All data were presented as means ± SD of at least three independent experiments. Values are significant at *P < 0.05, **P < 0.01 and ***P < 0.001 as indicated