Fig. 6
FcγR expression on tumor associated mononuclear phagocytes. a, Representative histograms showing FcγR expression on fresh donor matched peripheral blood (PB) and pleural fluid (PF) monocytes from a single mesothelioma patient. b, FcγRIIb expression (left) and FcγR A:I ratio (right) were quantified for PB and PF monocytes (FSChiCD45+CD14+ cells) sourced from mesothelioma patients using flow cytometry (n = 6 per group). c, Representative histograms showing expression of FcγRIIb on fresh monocytes isolated from lymphocele taken from 3 breast cancer patients. d, Representative histograms showing FcγRIIb expression on fresh donor matched renal monocytes (FSChiCD45+CD14+ cells) and macrophages (FSChiCD45+CD163+ cells) in normal kidney tissue and tumor from a single renal cell carcinoma (RCC) patient. e, FcγRIIb expression (left) and FcγR A:I ratio (right) were quantified for monocytes and macrophages sourced from normal kidney tissue and donor matched RCC specimens using flow cytometry (n = 5 per group). f, Representative histograms showing FcγRIIb expression on splenic and MCA205, CT26 and EG7 tumor associated CD11b+F4/80+ macrophages. g, Comparison of murine FcγRII expression (left) and FcγR A:I ratio (right) on CD11b+Ly6C+ monocytes and h, CD11blo/F4/80+ macrophages in recipient matched spleen and subcutaneous MCA205, CT26 and EG7 tumors (n = 5–9 per group). Each point on the graphs represents a unique human subject or mouse and bars represent group means. Statistical significance between groups was assessed using a paired two-tailed Wilcoxon test (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 and ns = non-significant). i, Immunofluorescence staining of hypoxic regions using hypoxyprobe (hypoxia probe) and anti-mouse FcγRII, on sections taken from a CT26 tumor. Localization of FcγRII expression in hypoxic regions is shown. Images representative of stained sections from 5 different mice. Scale bars 100 μm. Also see Additional file 6: Fig. S6