Fig. 2

Ectopic expression of circSCAP inhibits proliferation and migration of NSCLC cells in vitro. A The expression level of circSCAP in five NSCLC cell lines and one normal human bronchial epithelial cell line (16HBE) was evaluated by RT-PCR. B CircSCAP was stably overexpressed in A549 (left) and H1650 (right) cell lines. C-D CCK8 and colony formation assay were performed to investigate the effect of circSCAP overexpression on cell proliferation (C) and colony formation ability (D) of NSCLC cells. E–G Edu staining and flow cytometry was carried out to check the cell proliferation (E–F) and cell cycle (G) in NSCLC cells with circSCAP overexpression compared with control. H The protein level of G1 checkpoints involved in cell cycle of G1 transition to G2 were tested by immunoblotting. I-J The migration capability of NSCLC cells stably overexpressing circSCAP were evaluated through transwell assay. Data represent the mean ± SD of triplicate experiments and were analyzed by Student t test. * means P < 0.05. ** means P < 0.01, *** means P < 0.001