Fig. 3

Knock-down of circSCAP promotes proliferation and migration of NSCLC cells in vitro. A RT-PCR was performed to verify the knock-down efficiency of two circSCAP siRNAs. B Western blot indicated that circSCAP decrease exerted no effect on the level of linear SCAP (host gene of circSCAP). C-E CCK-8 and colony formation assays were performed to determine the proliferation (C) and colony formation ability (D-E) of NSCLC cells with circSCAP knock-down. F Flow cytometry was performed to check the effect of circSCAP decrease on cell cycle of NSCLC cells. G The protein level of G1 checkpoints involved in cell cycle of G1 transition to G2 were tested by immunoblotting. H-I Transwell assay was performed to determine the migration ability of NSCLC cells with circSCAP knock-down when compared with control. Data represent the mean ± SD of triplicate experiments and was analyzed by Student t test. * means P < 0.05. ** means P < 0.01, *** means P < 0.001