Fig. 3

Grade1 PDAC is closely surrounded by abundant CAFs. A Representative immunofluorescence images for αSMA (red), CK19 (green), and DAPI (blue) in Grade1 and Grade3 portions of human PDAC. Scale bars, (left) 100 µm and (right) 50 µm. B Representative immunohistochemistry images for αSMA in surgically resected PDAC tissue of indicated tumor grades. Scale bars, 200 µm. C Quantification of αSMA staining area as a percentage of the total area in PDAC tissue of indicated tumor grades (n = 15). Data are presented as the mean ± SD (***P < 0.001)