Fig. 3

SCAP is vital for sustaining sorafenib resistance. Knockdown of SCAP in sorafenib-resistant PLC/PRF/5 and MHCC-97H cells using a reverse siRNA transfection procedure. Immunoblot analysis of SCAP protein expression in PLC (A) and 97H (B) cells (n = 3). C The viability of sorafenib-treated PLC vector cells and SCAP knockdown cells (n = 3). Parental cells (NC), sorafenib-resistant cells (SR), SR vector cells (Vec) and SR SCAP knockdown cells (SCAPi) were treated with sorafenib (6 μm/L) for 24 h. D Clone assays of 4 groups in PLC and 97H cells (n = 3). Scratch-wound cell migration assays for each group of PLC (E) and 97H cells (F) (n = 3). Invasion assays for each group of PLC (G) and 97H cells (H) (n = 3). Bar = 100 μm. Flow cytometry analysis of the cell cycle (I) and apoptosis (J) of PLC cells after exposure to sorafenib (6 μm/L) for 24 h (n = 3). Data are the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001. P values were determined by Student’s t test in (A) and (B); repeated-measures ANOVA in (C) and (I); and one-way ANOVA test in (D), (E), (F), (G), (H), and (J)