Fig. 2

Anti-tumor effects of different reagents on tumor development and metastasis in an orthotopic model generated by 4T1 cells in Balb/C mice. A Growth curves of primary TNBC tumors. B Gross appearance of lungs harboring metastatic TNBC tumors. C Number of pulmonary metastatic tumors in mice derived from different groups. D Dose-dependent inhibitory effects of anti-PD-1 mAb on TNBC tumor growth in λ phage vaccinated mice as compared to the low dose group (12.5 μg), ^*** p < 0.001; ^****p < 0.000000001. E Dose-dependent inhibitory effects of anti-PD-1 mAb on pulmonary metastases in λ phage vaccinated mice. Comparisons were made to 4 different concentrations of anit-PD-1 mAb. F Percentage of immunosuppressive CD4⁺/CD25⁺/FOXP3⁺ Tregs among TILs in response to therapy. G-H Number of IFN-γ producing CD4⁺ and CD8⁺ T cells, respectively, in response to therapy. I Overall survival of mice harboring TNBC in response to therapy. ^*, p < 0.05; ^**, p < 0.01; ^***, p < 0.005; ^****, p < 0.001