Fig. 7

ZMAT1 correlates with p53 in Pancreatic Ductal Adenocarcinoma (PDAC). A BALB/c-nudes (n = 6 per group) were sacrificed 60 days after the injection and tumors dissected from respective groups were shown. B Tumor growth curves after the injection of SW1990/Vector cells and SW1990/ZMAT1-OV cells. Tumor volume was calculated every 10 days. C Tumor weight was measured in ZMAT1-OV and control groups. D The protein levels of ZMAT1, SIRT3 and p53 of tumors were analyzed by immunoblotting with the indicated antibodies. E IHC staining of ZMAT1, SIRT3, p53, Ki67 and Tunel in tumors from ZMAT1-OV and control groups. F Representative images of double-label immunofluorescence (IF) staining of ZMAT1 and p53 in 60 PDAC tissues. G IF staining showed ZMAT1 expression level highly correlated with p53 expression. H Kaplan–Meier analysis in PDAC patients grouped according to the expression levels of ZMAT1 and p53 showed that PDAC patients with high ZMAT1/high p53 expression had the longest overall survival among all the groups. I A schematic diagram for the role of the ZMAT1-SIRT3-p53 axis in regulation of cell cycle and apoptosis in PDAC. All * P-value < 0.05, ** P-value < 0.01, *** P-value < 0.001. Scale bars: 200 μm. P-values were assessed using two-tailed t-tests and ANOVA followed by Dunnett’s tests for multiple comparison in B-C. Spearman’s correlation was performed in G. Kaplan–Meier analyses and log-rank tests were conducted in H