Fig. 3

CD44v3 mimetic peptide 4 blocks the TrkA-CD44 association in MDA-MB-231 breast cancer cells. TrkA/CD44v3 (A) and TrkA/CD44v6 (B) complex associations were detected by the proximity ligation assay (PLA) in MDA-MB-231 breast cancer cells. Effects of a scramble peptide (SP) (C), CD44v3 mimetic peptide 3 (P3) and 4 (P4) (D) on TrkA/CD44v3 complex formation induced by NGF. Quantification of the PLA results was performed by ImageJ software (30 randomly chosen fields per condition of three different experiments). Statistical analyses were performed using one-way ANOVA followed by Bonferroni’s posttest. The error bars represent the standard error of the mean (S.E.M.); * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant