Fig. 3

OVOL2 inhibits proliferation, blocks cells in G2/M phase and impairs proper mitosis. A, B Proliferation assay of EV and OVOL2-expressing 8505c A and 8305c B cells. C Assessment of the ability of 8505c-OVOL2 to form colonies normalized on EV cells. Representative images show colonies at the experiment endpoint, 7 days from the seeding. D, E Cytofluorimetric analysis of 8505c cells after OVOL2 induction: cell cycle phases distribution D and specific % of cells in subG1 upon G0 synchronization E. Graphs represent means and SD of independent biological replicates *p < 0,05. F, G Frames extracted from real-time imaging videos showing altered morphology of 8505c F and 8305c G 48 and 72 h upon OVOL2 induction compared to EV cells. H Frames sequence showing the time requested from EV and OVOL2-induced cells to complete cell division. White arrowheads divide subsequent frames, light blue arrowheads indicate abscission and midbodies. For 8505c EV, 10 consecutive frames are displayed. For 8505c OVOL2 one frame every 3 is displayed, for a total of 27 frames. For 8305c EV, frame 1 and 4 to 12 consecutive frames are reported. For 8305c OVOL2 frame 1 and every 3 frames from 9 to 33 are shown. Consecutive frames are obtained at 5 min from each other