Fig. 2

Analysis of hOA-DN30 MET inhibitory properties. a A549 cells treated with increasing concentrations of hOA-DN30 for 72 h. b A549 cells treated with 1 μM hOA-DN30 for the indicated times. c A549 cells treated with 1 μM hOA-DN30 for 48 h and then left untreated for the indicated times. d GTL-16 cells treated with increasing concentrations of hOA-DN30 for 24 h. MET, AKT, ERK protein levels and activation were determined by Western blot analysis of cell lysates. MET ectodomain levels were determined by Western blot analysis of cell culture supernatants. To normalize protein loading, filters were re-probed with anti-Hsp90. p145 MET: MET receptor β chain; p145 P-MET: phosphorylated MET receptor β chain; p80 MET: MET-ECD; p60 AKT: AKT; p60 P-AKT: phosphorylated AKT; p44/42 ERK: ERK; p44/42 P-ERK: phosphorylated ERK; Hsp90: Heat shock protein. Data reported in the figure are representative of at least two experiments