Fig. 7

Exosomes enhance tumor angiogenesis by suppressing CXCL10 in endothelial cells. A RNA-seq was performed to detect the downstream pathways and differentially-expressed genes of HUVECs co-cultured with PABPC1-overexpressing ESCC cells. B The differentially-expressed genes are shown, and expression of selected genes was detected using qRT-PCR. C Detection of CXCL10 by western blotting. D Detection of CXCL10 by ELISA. E Predicted miR-21-5p target sequences in the 3’UTRs of CXCL10 mRNA. Relative CXCL10 reporter activities were detected in 293 T cells co-transfected with miR-21-5p and luciferase reporters. F Expression of miR-21-5p was detected after miR-21-5p transfection. G Transwell invasion and capillary tube formation assay of HUVECs treated with the indicated transfection (scale bar: 50 µm). H Proposed model illustrating the modulatory role of PABPC1 in promoting ESCC tumorigenesis. Data represent the mean ± SD of 3 separate determinations. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t test