Fig. 7

Circ-MTHFD1L maintains gemcitabine resistance through miR-615-3p/RPN6 axis. A-F The proliferative ability of the indicated cells dealing with gemcitabine was investigated via colony formation assays (A-F) and CCK-8 assays (cell viability and IC50) (E-F). Representative colony formation images are shown (A, C), and the numbers of colonies were summarized (B, D). G Flow cytometry analysis of the cell cycle progression of the indicated cells dealing with gemcitabine was performed. I Confocal microscopic analysis of γH2AX staining and the comet assay in the indicated cells was evaluated. The quantification of the results is presented. Data are shown as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001, between the indicated groups. J The RAD50, RAD51, BRCA1, BRCA2, PCNA, P53 and P21 protein levels in PANC-1-GR and BxPC-3-GR cells from different groups were determined by western blot analysis. GAPDH was used as a loading control