Fig. 1

Identification of candidate regulators of OS cell resistance to Resistance. A Schematic diagram illustrates the workflow of genome-wide CRISPR/Cas9 knockout library screening. B The nine-quadrant diagram shows sgRNA sequencing results of doxorubicin treatment for 14 days. C SgRNA distribution of Top10 genes in negative screening. D GO enrichment analysis and E KEGG enrichment analysis of negative screening results of day 14. F Gene Set Enrichment Analysis (GSEA) showed that apoptosis and DNA double strand break processing pathway were influenced of negative screening on day 14. G Venn diagram shows differentially expressed genes in four groups described as: doxorubicin-treated group (DOX), DMSO-treated group (DMSO), none treated group (baseline). 28 genes were enriched. H Dynamic distribution of sgRNA Read counts of enriched genes