Fig. 1
Hypoxic TME increases Cis-Pt resistance and CA IX/XII expression levels in HNSCC. A-B Cell viability analysis by MTT assay of FaDu and SCC-011 cells grown in normoxic (21% O2) and hypoxic (1% O2) conditions and incubated with increasing concentrations of Cis-Pt (0–10 µM) for 72 h. All the data are expressed as percentage of viable cells, considering the untreated control cells as 100%. Bars depict mean ± SD of three independent experiments. (***p < 0.001; **p < 0.01; *p < 0.1). C HIF-1α, CA IX and CA XII protein levels were analyzed by western blot analysis in FaDu and SCC-011 cell lines grown under normoxia (21% O2) and hypoxia (1% O2) for 72 h. Actin and GADPH were used as loading control. Representative data from one of three experiments are shown. D For CA IX and CA XII mRNA expression analysis in HNSCC patients the Genomics Analysis and Visualization platform (R2: Genomics analysis and visualization platform; http://r2.amc.nl) was used. The analysis was performed with the following datasets: GSE18674 which includes 22 human normal tissues and GSE42743 which includes 103 oral cavity tumors. The correlation was assessed by one-way analysis of variance (ANOVA), through the R2 platform and presented in box plots. (***p < 0.0001)