Fig. 6
Effect of combinatorial treatment with SLC-0111 and Cis-Pt on STAT3, AKT, ERK and EMT program activation. A-B Analysis by western blot of expression levels of pSTAT3/STAT3, pAKT/AKT and pERK/ERK. C-D Analysis by western blot of expression levels of epithelial (E-Cadherin) and mesenchymal markers (N-Cadherin and β-Catenin). FaDu and SCC-011 cells were grown under hypoxia (1%O2) and treated with Cis-Pt (1 µM), SLC-0111 (100 µM) and combination of the two drugs for 72 h. Equal loading was confirmed by immunoblot with anti-GADPH antibody. Representative images are shown