Fig. 7From: Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progressionSETD3 promotes lung cancer cell proliferation and migration in A549 cells through YBX1/EVs-hY4F axis. A Levels of SETD3 protein in tissue from LUAD cancer patients or control subjects according to the CPTAC database (P = 0.0387). B Pearson correlation analysis of plasma EV-hY4F and tumor tissue SETD3 expression levels in lung adenocarcinoma (LUAD) patients (n = 24) detected by qPCR. Transcript levels of SETD3 were normalized by internal control 18S rRNA, and plasma hY4F levels were normalized by external control cel-miR-39-3p. C EdU assay was performed to assess the effect of SETD3 knockdown on A549 cell proliferation (at 24 h after transfection). D Flow cytometry using Annexin V-FITC/PI staining was performed to analyze apoptosis of A549 cells transfected with SETD3 siRNA (at 24 h after transfection). The effect of SETD3 knockdown on A549 (E) migration and (F) invasion was examined by transwell assay (at 24 h after transfection). G EdU assay was performed to assess the effect of YBX1 co-transfection with siRNA targeting SETD3 on A549 cells (at 48 h after transfection). H Colony formation assay with YBX1 and siSETD3 co-transfected A549 cells (at 7 d after transfection). Data from three independent experiments are shown as the mean ± SD (error bars). *P < 0.05, **P < 0.01, ***P < 0.001 (Student’s t-test). NC: negative controlBack to article page