Fig. 6

IL-32 in ESCC-derived EV promotes M2 macrophage polarization via FAK-STAT3 activation. A Enrichment from GSEA revealed IL-32 expression positively correlated with the inflammatory response and IL-6-JAK-STAT3 signaling pathway in the GSE23400 dataset. B The GSEA analysis of EC109 shNC and EC109 shIL-32. C Western blot analysis of the expression of STAT1, STAT3, p-STAT1 (Y701) and p-STAT3 (Y705) in MDMs treated with EV. D The expression of p-FAK (Y576/Y577) and p-STAT3 (Y705) in MDMs treated with EV were detected by Western blot. E EV cocultured with MDMs treated with FAK inhibitor (Y15) at 2 µM for 12 h, and Western blot determined the phosphorylation of FAK and STAT3 in MDMs. Each band is representative of three independent experiments