Fig. 3

mMSCs-Exo alleviated radiation-induced lung injury (RILI) and fibrosis in vivo. A H&E and Masson staining in lung tissues from the mouse model of RILI with or without mMSCs-Exo at the indicated time points post-irradiation, n = 4 mice per group. Representative images were presented. Scale bars = 200 μm. B Percentage of collagen deposition in lung sections was quantified from different groups at 12 w post-irradiation. C Biochemical assay for Hydroxyproline content in lung sections from different groups at 12 w post-irradiation, shown as microgram of Hyp per milligram of wet weight (μg/mg). D, E ELISA assay for proinflammatory cytokine IL-1β (D) and IL-6 (E) in serum from different groups at the indicated time points post-irradiation. F Representative images of IHC assay for E-cadherin and Vimentin proteins from different groups at 12 weeks post-irradiation, Scale bars = 100 μm. Data are presented as the mean ± SD. from three independent experiments, * p < 0.05