Fig. 5

miR-466f-3p-dependent AKT/GSK3β pathway was critical for radiation-induced EMT in MLE-12 cells. A The overlap of potential targets of miR-466f-3p predicted by Targetscan, miRmap, PITA, and miRanda databases. B KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis for a panel of 367 overlapped targets of miR-466f-3p, and PI3K/AKT pathway was included among the top 10 enrichments. C, D Western blot analysis (C) and densitometric quantification (D) of p-AKT, p-GSK3β, and SNAIL in nonirradiated control and irradiated cells with or without AKT inhibitor (LY294002) at 1 h post-irradiation. E, F Western blot analysis (E) and densitometric quantification (F) of E-cadherin and Vimentin in irradiated cells with or without AKT inhibitor at 48 h post-irradiation. G-J Western blot analysis and densitometric quantification of p-AKT, p-GSK3β, and SNAIL (G, H), E-cadherin and Vimentin (I, J) in irradiated MLE-12 cells with scramble and miR-466f-3p mimic at 1 or 48 h post-irradiation. All the above western blot analysis used AKT, GSK3β, or GAPDH as the loading control. K Immunoflurescence staining for SNAIL (green) and DAPI (blue) in irradiated MLE-12 cells with scramble and miR-466f-3p mimic at 48 h post-irradiation. Data are presented as the mean ± SD. from three independent experiments, * p < 0.05