Fig. 3

CRABP1/CRABP2 rebalances in RA-resistant neuroblastoma after BGA002-RA treatment. A boxplot represents the mRNA expression of CRABP1. Each boxplot represents the mRNA expression for a cancer type as listed in the Cancer Cell Line Encyclopedia. Each dot represents the mRNA expression for a single cell line, the middle line represents the median, the outer edges of the box indicate the first and the third quartiles, and the whiskers specify samples within 1.5 times the interquartile range. B mRNA expression of CRABP1 and CRABP2 in an neuroblastoma dataset (E-MTAB-1781). The violin plot represents normalized (z-score) mRNA expression for non-MNA and MNA patients. Each dot represents an individual sample; the middle line indicates the median. Statistic: Wilcoxon matched-pair test. C Kaplan–Meier plots for the probability of overall survival over time for neuroblastoma patients (E-MTAB-1781). The dark grey line indicates patients with normalized gene expressions higher than 1 (z-score > 1). The p-value is indicated in the middle (Log-rank test). D a heatmap of the gene expression variation after 12 hours of treatment in neuroblastoma cell lines. Columns represent cell lines (grouped according MYCN amplification and p53 mutation status), rows represent CRABP1 and CRABP2, color scale represents the log₂ fold change over the control (untreated). The grey color indicates unexpressed genes. (MNA cell-lines: KELLY, CHP-134, IMR-32, SMS-KAN, LS, MHH-NB-11, NGP, SIMa, GI-LI-N; MNA-p53mut: SK-N-B [2] c, NMB, LAN-1; single copy: NBL-S, LAN-6, SH-SY5Y; single copy and p53mut: SK-N-F1) E Cell viability after 72 hours of treatment in SK-N-BE [2] c cells (n = 3 different biological replicates). SK-N-BE [2] c cells is MYCN amplified, p53 mutated. Where indicated in the figure: *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001