Fig. 9

Overexpression of circ_0087429 in vivo can inhibit the progression of cervical cancer a. Image of subcutaneous tumour tissues in the circ_0087429-overexpressing group and control group. b-c. Volume and weight of subcutaneous tumour tissues in each group. d. The expression levels of circ_0087429, miR-5003-3p and OGN in subcutaneous tumour tissues of each group were detected by qRT-PCR. e–f. The expression levels of OGN, N-cadherin and E-cadherin in subcutaneous tumour tissues of each group were detected by western blotting. g-h. The expression levels of OGN, N-cadherin, E-cadherin, Ki67 and CD31 in subcutaneous tumour tissues of each group were detected by immunohistochemical staining. Scale bar, 30 μm. i-l. Representative images and bar graphs of liver (i-j) and lung (k-l) metastases with circ_0087429-overexpressing group and control group in a nude mouse metastatic tumour model. Metastatic nodules are indicated by arrows. Scale bar, 60 μm. m. A schematic diagram showing how circ_0087429 regulates the expression of OGN by sponging miR-5003-3p and thereby reverses EMT. MET: mesenchymal to epithelial transition. *p < 0.05, **p < 0.01, ***p < 0.001