Fig. 6

The expression and oncogenic role of JMJD5 in glycolysis and proliferation of GBM. A The localization of HEATR5B-881aa in U251 and U373 cells by IF assays. Green, HEATR5B-881aa; blue, DAPI nuclear staining. Scale bars, 10 μm. B The expression of JMJD5 protein in NBTs, LGGTs, and HGGTs groups. Data are presented as the mean ± SD (n = 9, each group). ^***P < 0.001 vs. NBTs group; ^###P < 0.001 vs. LGGTs group by one-way ANOVA. C The expression of JMJD5 protein in NHA, U251, and U373 cells. Data are presented as the mean ± SD (n = 3, each group). ^***P < 0.001 vs. NHA group by one-way ANOVA. D The effects of JMJD5 on pyruvate kinase enzymatic activity in U251 and U373 cells. E and F The effects of JMJD5 on glucose consumption (E) and lactate production (F) in U251 and U373 cells. G The effects of JMJD5 on ECAR in U251 and U373 cells. H The effects of JMJD5 on proliferation in U251 and U373 cells. Data are presented as the mean ± SD (n = 3, each group). ^**P < 0.01, ^***P < 0.001 vs. sh-JMJD5-NC group; ^###P < 0.001 vs. OV-JMJD5-NC group by one-way ANOVA. I The expression of JMJD5 protein in response to HEATR5B-881aa upregulation or downregulation in U251 and U373 cells. Data are presented as the mean ± SD (n = 3, each group). ^***P < 0.001 vs. OV-HEATR5B-881aa-NC group; ^##P < 0.01 vs. circHEATR5B group by one-way ANOVA. J-L The effects of HEATR5B-881aa and JMJD5 on pyruvate kinase enzymatic activity (J), glucose consumption (K), and lactate production (L) in U251 and U373 cells. M The effects of HEATR5B-881aa and JMJD5 on ECAR in U251 and U373 cells. N The effects of HEATR5B-881aa and JMJD5 on proliferation in U251 and U373 cells. Data are presented as the mean ± SD (n = 3, each group). ^**P < 0.01, ^***P < 0.001 vs. OV-HEATR5B-881aa + OV-JMJD5-NC group by one-way ANOVA