Fig. 7

Knockdown of NLRP3 notably weakened proliferation and NLRP3 inflammasome activation in IL-6-mediated OSCC cells. NLRP3 was silenced in SCC-15 and SCC-25 cells, which were addressed with 25 ng/ml IL-6. NLRP3 expression was assessed through RT-qPCR (A) and western blot (B). Cell proliferation was determined via (C) CCK-8, (D) EdU staining and (E) colony formation. (F) The expressions of the inflammation-related proteins were monitored with Western blot. (G) ELISA kits were applied to identify IL-1β and IL-18 levels. *** P < 0.001 vs. IL-6 group; ### P < 0.001 vs. IL-6 + shCTRL group