Fig. 4

E2F target signature is depleted in cells overexpressing miR-183-5p|+2. a Mass spectrometry derived data from MDA-MB-231 cells overexpressing one of the 5’isomiRs of miR-183-5p were compared for differential protein expression (Student’s T-Test). Non-imputed, z-scaled expression of proteins with significantly different expression between samples are visualized as heatmap. b Gene set enrichment analysis was performed on the imputed proteome data for each of the overexpression cell lines using preranked GSEA and log2 foldchange as ranking metric. Normalized enrichment score (NES) and adjusted p-value of the respective gene set was used to visualize results in a bubble heatmap. c Heatmap showing z-scaled protein expression of all proteins within the gene set E2F targets for the samples overexpressing one of the isomiRs of miR-183-5p. Non-imputed proteome data were used. d Venn diagram showing the overlap between target prediction results of miR-183-5p and its 5’isomiRs. Consensus predictions of custom target predictions using both Miranda and TargetScan prediction algorithms were used