Fig. 4

AR binds to the DHX9 promoter and modulates its expression. A The plot shows the Pearson correlation between AR and DHX9 expression in 499 patients with PC (TCGA, Firehose legacy project). B qPCR analyses of ChIP experiments performed in LNCaP cells using AR antibody and anti-IgG rabbit Abs (IgG). Associated DNA was expressed as % of input. The sequences tested for the AR binding on the DHX9 promoter are indicated. LNCaP cells were cultured in CSS for 48 hrs, stimulated with DHT (10 nM) for 24 hrs and the expression of DHX9 was measured by qPCR (C) or WB analysis (D). Quantification of mRNA and protein level are shown in the bar graphs as the mean ± SD of three independent experiments, considering the control sample (CSS) as 1. LNCaP cells were treated with Enzalutamide for 24 hours (1 μM) and DHX9 expression was measured by qPCR (E) or WB analysis (F). Histogram represents the mean ± SD of three independent experiments, considering the DMSO sample as 1. Statistical analyses were performed by Student’s t test, p values: *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001