Fig. 6
Expression of PHGDH in wild type (control) and KRASG12D expressing (MutKRAS) Huh28 cells, response to L-serine availability. a PHGDH mRNA levels in control and MutKRAS Huh28 cells grown in complete medium (t = 0) and at the indicated time-points after L-serine depletion. *p < 0.05 vs control. b PHGDH protein levels were analyzed by western blotting in same samples described in A. Representative blots, including HSP90 analysis as loading control, are shown. c Growth of control and MutKRAS Huh28 cells in L-serine depleted medium referenced to cell growth in complete medium. *p < 0.05, **p < 0.01