Fig. 7
G9a inhibition blunts the adaptive upregulation of PHGDH expression to L-serine availability in CCA cells. a Control and KRASG12D expressing (MutKRAS) Huh28 cells were grown in complete medium for 60 h with or without CM-272 (200 nM) and then maintained in complete medium or without L-serine for another 20 h. At this point PHGDH mRNA expression was analyzed. *p < 0.05. b PHGDH protein levels were analyzed by western blotting in same samples described in a. Representative blots, including HSP90 analysis as loading control, are shown. c UPLC-ToF-MS analysis of [U-13C] glucose flux into serine in control and MutKRAS Huh28 cells treated of not with CM-272 ( 200 nM, 66 h). *p < 0.05. d ATF4 protein levels were analyzed by western blotting in same samples described in a. Representative blots, including HSP90 analysis as loading control, are shown