Fig. 4
From: Essential role for STAT3/FOXM1/ATG7 signaling-dependent autophagy in resistance to Icotinib
STAT3/FOXM1/ATG7 signalling is essential for icotinib resistance. A Immunoblotting was performed to determine the impact of FOXM1 blockade on protein expression of ATG7. B-D Representative results of protein changes of FOXM1 and ATG7 levels (B), autophagic flux with the mRFP-GFP-LC3 reporter (C), and cell apoptosis (D) in icotinib-treated cells in response to FOXM1 knockdown with two specific siRNAs against FOXM1. E Expression of ATG7 was detected in icotinib-treated cells transfected with STAT3-CA and siFOXM1 alone or combination. [***P < 0.001, ns, no significance as compared with the DMSO group. ###P < 0.001 as compared with the icotinib group. ζζζP < 0.001 as compared with icotinib plus STAT3WT and siNC. ξξξP < 0.001 as compared with icotinib plus STAT3-Y705F and siNC]