Fig. 8

FOXO4 regulate circPDE5A expression in prostate cancer cells. A, Venn diagram showing transcriptional factors predicted using JASPAR and PROMO databases. B, The expression of circPDE5A and PDE5A mRNA in FOXO4 knockdown 22Rv-1 cells. C, The expression of circPDE5A and PDE5A mRNA in FOXO4 overexpression 22Rv-1 cells. D, Top: the putative FOXO4 binding motif in PDE5A’s promoter region predicted using the JASPAR database; bottom: the relative enrichment of FOXO4 in three potential binding sites compared with IgG. E, The effect of FOXO4 on the luciferase activity of PDE5A’s promoter region using a dual-luciferase reporter assay. F, Relative expression levels of FOXO4 in 50 paired prostate cancer tissues and adjacent normal tissues. G, Correlation analysis of the expression between FOXO4 and circPDE5A in PCa specimen. H, The schematic diagram illustrating the role of circPDE5A in prostate cancer .Data represents mean ± S.D. from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001