Fig. 6
FLNA is upregulated by S100A8/A9-RAGE-FAK-YAP transduction network in TNBC. A FLNA mRNA levels in MDA-MB231 cells overexpressing RAGE and treated for 6 hours with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM RAGE antagonist FPS-ZM1, 1 μM FAK inhibitor VS-4718 and 1 μM YAP/TEAD disruptor Verteporfin. B FLNA (red) and Nuclei (blue) confocal immunofluorescence staining in MDA-MB231 cells overexpressing RAGE and stimulated for 6 hours with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM RAGE antagonist FPS-ZM1, 1 μM FAK inhibitor VS-4718 and 1 μM YAP/TEAD disruptor Verteporfin, respectively. C Relative percentage of FLNA fluorescence intensity. Error bars represent mean ± SD. * indicates p-value < 0.05. Results shown are representative of three independent experiments performed in triplicate