Fig. 8

S100A8/A9-RAGE system increases the migration of TNBC cells. A-B Representative images (left) and relative quantification (right) of transwell migration in MDA-MB231 cells overexpressing RAGE and treated for 4 hours with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM RAGE antagonist FPS-ZM1. C-D Representative images (left) and relative quantification (right) of transwell migration in MDA-MB231 cells overexpressing RAGE and treated for 4 hours with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM FAK inhibitor VS-4718. E-F Representative images (left) and relative quantification (right) of transwell migration in BT-549 cells overexpressing RAGE and treated for 4 hours with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM RAGE antagonist FPS-ZM1. G-H Representative images (left) and relative quantification (right) of transwell migration in BT-549 cells overexpressing RAGE and treated for 4 hours with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM FAK inhibitor VS-4718. I-J Representative images (left) and relative quantification (right) of transwell migration in BT-549 cells overexpressing RAGE transfected with siRNA control (20 nM) and siRNA targeting FLNA (20 nM) and stimulated for 4 hours with 100 ng/ml rhS100A8/A9. K FLNA knockdown efficiency is shown. Error bars represent mean ± SD. * indicates p-value < 0.05. Results shown are representative of three independent experiments performed in triplicate