Fig. 6
Combined GR and ERα-expression resulted in reduced angiogenesis and tumor burden. A Tumor volume in mice with tumors derived from BE(2)-GR + EV (n = 5) and BE(2)-GR + ERα (n = 6) cells. Growth was followed until the control group reached the ethical endpoint volume of 1 cm3. Data is presented as mean ± SD. Statistical analysis: Mann-Whitney t-test with * and ** indicating p < 0.05 and p < 0.01, respectively. B Tumor weight at experimental endpoint. Data is presented as mean ± SD. Statistical analysis: t-test with * and ** indicating p < 0.05 and p < 0.01, respectively. C Immunohistochemistry analysis of BE(2)-GR + EV and BE(2)-GR + ERα xenograft tumors stained with anti-Ki67, anti-GR, or anti-ERα antibodies. Scale bars indicate 50 μm. D Immunohistochemistry analysis of BE(2)-GR + EV and BE(2)-GR + ERα xenograft tumors stained with anti-p75NTR, anti-SCG2, or anti-βIII-tubulin antibodies. Scale bars indicate 50 μm. E Photos of tumors derived from BE(2)-GR + EV or BE(2)-GR + ERα cells with ruler. Images of immunohistochemistry analysis of the respective xenograft tumors stained with anti-endomucin for visualization of blood vessels. Scale bars indicate 100 μm. Representative images from at least five independent stainings per condition (C-E)