Fig. 2

DUB1 facilitates Hippo/TAZ axis activity in gastric cancer cells A: DUB1 depletion reduced the TAZ protein level in MGC803 cells. MGC803 cells were transfected with siControl or siDUB1. After 48 h, the cells were harvested for western blot analysis. DUB1 and TAZ protein levels were determined by Western blotting. Actin was used as the internal control. B: DUB1 depletion reduced the TAZ protein level in AGS cells. AGS cells were transfected with siControl or siDUB1. After 48 h, the cells were harvested for western blot analysis. DUB1 and TAZ protein levels were determined by Western blotting. Actin was used as the internal control. C: DUB1 depletion decreased Hippo target gene expression in MGC803 cells. MGC803 cells were transfected with siControl or siDUB1. After 48 h, total RNA was extracted for gene expression analysis. Each group was tested in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 for comparisons of target gene expression. D: DUB1 depletion decreased Hippo target gene expression in AGS cells. AGS cells were transfected with siControl or siDUB1. After 48 h, total RNA was extracted for gene expression analysis. Each group was tested in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 for comparisons of target gene expression. E: DUB1 depletion decreased TEAD luciferase activity in MGC803 cells. MGC803 cells were transfected with siControl or siDUB1. After 24 h, the cells were transfected with TEAD luciferase reporter plasmids. After another 24 h, the cells were harvested for luciferase activity analysis. F: DUB1 depletion decreased TEAD luciferase activity in AGS cells. AGS cells were transfected with siControl or siDUB1. After 24 h, the cells were transfected with TEAD luciferase reporter plasmids. After another 24 h, the cells were harvested for luciferase activity analysis. G: DUB1 overexpression increased the TAZ protein level in HEK293 cells. HEK293 cells were transfected with 2 µg of the Flag-DUB1 or Flag vector. After 48 h, the cells were harvested for western blot analysis. DUB1 and TAZ protein levels were determined by Western blotting. Actin was used as the internal control. H: DUB1 overexpression increased Hippo target gene expression in HEK293 cells. HEK293 cells were transfected with 2 µg of the Flag-DUB1 or Flag vector. After 48 h, total RNA was extracted for gene expression analysis. Each group was tested in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 for comparisons of target gene expression. I: DUB1 overexpression increased TEAD luciferase activity in HEK293 cells. HEK293 cells were transfected with 2 µg of the Flag-DUB1 or Flag vector. After 24 h, the cells were transfected with TEAD luciferase reporter plasmids. After 24 h, the cells were harvested for luciferase activity analysis