Fig. 6

NCAPD3 enhanced tumor growth and aerobic glycolysis in a xenograft tumor mice model. A, F Tumor images dissected from the nude mice after being subcutaneously injected with HCT116 cells or SW480 cells, followed by treatment with intraperitoneal injection of PBS or 2-DG. a represents Lv-NCAPD3 + 2-DG group, b represents Lv-NCAPD3 group, c represents Lv-control group, d represents shcontrol group, e represents shNCAPD3 group, f represents shcontrol + 2-DG group, g represents shNCAPD3 + 2-DG group, n = 5. Scale bar: 1 cm. B, G Average tumor volume and weight of each mice group were measured and presented. C, H Relative lactate levels in xenograft tumor with indicated treatment were tested and presented. D, I Expression of proteins related to glycolysis and TCA cycle were detected in xenografts after indicated treatment by Western blot. 1, 2 represent tumor tissues from different mice. E, J Representative images of IHC staining of NCAPD3, c-Myc, E2F1 and Ki-67 on tumor sections. Scale bar: 20 μm. shCTRL represents shcontrol, shNC3 represents shNCAPD3. Statistical results were shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001, based on two-way ANOVA or Student’s t-test