Fig. 7

Knockout of NCAPD3 attenuated colorectal carcinogenesis in AOM/DSS-induced mice model. A Body weight changes of wild type (WT) and NCAPD3^± mice during AOM/DSS treatment (n = 5). B Typical intestines of mice on day 108 were dissected and photographed after mice were euthanized. Scale bar: 1 cm. Colorectal lengths of WT and NCAPD3^± mice were measured (n = 5). C Representative images of colorectal tumors from WT and NCAPD3^± mice (n = 5). D Expression of proteins related to glycolysis and TCA cycle were detected in tumor sections from WT and NCAPD3^± mice by Western blot. 1, 2, 3 represent tumor tissues from different mice E Histological analysis and representative IHC images of c-Myc, E2F1, GLUT1, Ki67 on the tumor sections from WT and NCAPD3.^± mice. Scale bar: 20 μm. Results were shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t-test or two-way ANOVA