Fig. 4

RYR1 depletion suppresses mETC gene expression. A Heatmaps showing relative expression levels of mitochondrial proteins measured by RPPA in ARK1-shCtrl and shRYR1 cells (left); or cells treated with vehicle or dantrolene (right). Signal intensities were normalized to the control groups. B Graphic illustration of components in the 4 complexes of mitochondrial respiration chain. C, D. Relative mRNA (C) and protein (D) expressions of mETC genes determined by qPCR and Western blot, respectively, in USC cells transfected with RYR1-specific shRNAs or shCtrl. β-actin was used as a loading control. Data are shown as the mean ± SEM of 3 independent experiments. E Relative mRNA expression of mETC genes determined by qPCR in USC cells treated with 50 μM dantrolene or vehicle. Data are shown as the mean ± SEM of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., no significance (Student t-test). F Relative expressions of NDUFB4, SDHA, COXIV and ATP5a in TCGA dataset (Student t-test). G Correlation of NDUFB4, SDHA, COXIV and ATP5a with RYR1 in GSE24537