Fig. 3
From: CD147 mediates epidermal malignant transformation through the RSK2/AP-1 pathway
Epidermal CD147 regulates the recruitment of MDSCs under inflammation conditions. A-B The percentage of CD45+ cells is elevated in EpiCD147-OE mice. Transgenic mice were treated with TPA for 12 h, and skin lesions were subjected to flow cytometry analysis. The gating strategy (A) and bar charts of the percentage of CD45+ cells (B) were presented. C-D The percentage of infiltrated CD11b+Gr1+ MDSCs was elevated in EpiCD147-OE mice. The gating strategy (C) and bar charts of the percentage of CD11b+Gr1+ cells (D) were presented. E-F CXCL1 was increased in EpiCD147-OE mice. RT-PCR (E) and ELISA (F) of CXCL1 were performed with TPA-treated skin lesion. G-H Schematic diagram of primers for genotyping and targeting strategies. EpiCD147-KO genotyping was carried out with Primer 3 (P3) and Primer 4 (P4) (G). A 272 bp band was assessed for EpiCD147-KO genotyping (H left panel), and DNA samples were prepared from total skin, and the expression of CD147 was test by q-PCR (H right panel). I-J The percentage of CD45+ cells was reduced in EpiCD147-KO mice compared to EpiCD147-WT mice. The gating strategy (I) and bar charts of the percentage of CD45+ cells (J) were presented. K-L The percentage of infiltrated CD11b+Gr1+ MDSCs was reduced in EpiCD147-KO mice. The gating strategy (K) and bar charts of the percentage of CD11b+Gr1+ cells (L) were presented. M-N CXCL1 was decreased in EpiCD147-KO mice compared to control mice. RT-PCR (M) and ELISA (N) of CXCL1 was performed with TPA-treated skin lesions. All data were presented as the mean ± SD. The significance of differences was evaluated using Student’s t-test