Fig. 6
From: CD147 mediates epidermal malignant transformation through the RSK2/AP-1 pathway
D207-230 silencing blocked the interaction of CD147 with RSK2 and inhibited cell growth and transformation. A-B RSK2 binds to CD147. 293 T cells were cotransfected with RSK2-c-Myc and CD147-V5 plasmids. Co-IP was performed with anti-c-Myc (A) or anti-V5 (B) antibodies, followed by immunoblotting with the indicated antibodies. C RSK2 is inhibited in CD147-knockdown keratinocytes. Membrane protein and whole-cell lysates of sh-Mock or sh-CD147 HaCaT cells stimulated with EGF were extracted and subjected to immunoblot analysis using indicated antibodies. ATP1A1 and GAPDH were used as loading control. D-E The CD147D207-230 mediates the interaction between CD147 and RSK2. Schematic diagram of truncated CD147 (D), and 293 T cells were cotransfected with RSK2 and CD147-c-Myc or CD147D207-230-c-Myc plasmids. Co-IP was performed with anti-c-Myc antibodies, followed by immunoblotting with the indicated antibodies (E). F CD147D207-230 attenuates EGF induced RSK2 signaling pathway. Membrane protein and whole-cell lysates of JB6-Mock, −CD147, and- CD147D207-230 cells stimulated with EGF were extracted and subjected to immunoblot analysis using indicated antibodies. ATP1A1 and GAPDH were used as loading control. G-J The CD147D207-230 blocks the pro-migration and -invasion ability in JB6 cells. JB6 transfected with Mock, CD147 and D207-230 were subjected to wound healing assay (G), migration (H upper panel, I) and transwell assay (H lower panel, J)