Fig. 7

YAP-TEAD complex activation is involved in FGFR2⁺ fibrocyte differentiation. A. The expression levels of total YAP and inactive YAP (pYAP-S127/Y357) were detected in NF, CAF, Fbc and iCAF from 3 ESCC patients by western blots. β-actin was used as loading control. B. Representative IF images of subcellular localization of YAP in NF (FGFR2⁻ Fibronectin^strong), CAF (FGFR2⁺Fibronectin^strong), Fbc (FGFR2⁺Fibronectin^weak) and iCAF (FGFR2⁺Fibronectin^strong). Nuclei were counterstained with DAPI. C. GSEA analysis of multiple TEAD and YAP/TAZ gene sets was performed on our RNA-seq data (C1 vs F1 and C2 vs F2). The normalized enrichment score, nominal p-value and the sources of gene sets are summarized in the table. D. Expression of indicated genes in YAP/TEAD knockdown CAFs were measured by qPCR. The data are represented as mean ± SD of the fold reduction compared to scramble control (shCtrl). The experiment was repeated three times. Abbreviations: GSEA, gene set enrichment analysis; NF, normal fibroblast; CAF, cancer-associated fibroblast; Fbc, fibrocyte; iCAF, induced-cancer-associated fibroblast; qPCR, semi-quantitative PCR; IF, immunofluorescence; ESCC, esophageal squamous cell; Ctrl, control