Fig. 1
Expression of C1GALT1 and T antigen in BLCA. A T antigen production by C1GALT1. B, C Differential C1GALT1 expression (B) and T antigen levels (C) in BLCA, by TMA analysis. Representative immunohistochemical results for paired clinical tissues are shown. ***, p < 0.001. D Representative western and lectin blotting results for C1GALT1 and T antigen levels in BLCA (T) and adjacent normal (A) tissues. *p < 0.05; ***, p < 0.001. E T antigen levels in BLCA patient sera, by ELISA. *, p < 0.05. F mRNA expression of C1GALT1 in paired and unpaired BLCA and adjacent normal tissues in TCGA database. **, p < 0.01. G mRNA expression of C1GALT1 in BLCA patients from GSE27448 series. **, p < 0.01. H, I Overall (H) and disease-free survival (I) of dichotomized C1GALT1 mRNA expression in BLCA patients in TCGA database, using GEPIA platform. J Left: representative ESI-MS/MS spectra of O-glycan structures in HCV29 and YTS-1 cells. Right: relative abundance of T-antigen in normal epithelial bladder cells HUC-1 and HCV-29 vs. in bladder cancer cells KK47, T24 and YTS-1