Fig. 3
AGK regulates the G1-S-phase transition in EOC cells. A Representative micrographs (left panel) and the quantification (right panel) of EdU incorporation in the OVCAR3/CAOV3-AGK as well as vector control cells. B Cell cycle flow cytometric analysis of AGK-overexpressing and vector control cells. C Representative micrographs (left panel) and the quantification (right panel) of EdU incorporation in the OVCAR3/CAOV3-AGK-RNAi as well as vector control cells. D Cell cycle flow cytometric analysis of OVCAR3/CAOV3-AGK-RNAi and vector control cells. AGK altered the expression of G1-S-phase cell-cycle regulators. Western blotting (E) and real-time PCR (F) analysis of the expression of p21Cip1, p27Kip1, cyclin D1, p-Rb and the total Rb protein in the OVCAR3/CAOV3-AGK as well as OVCAR3/CAOV3-AGK-RNAi cells; α-Tubulin was used as a loading control. Each bar represents the mean ± SD of 3 independent experiments. *P < 0.05. N = 3 independent experiments. G GSEA plot analysis showed that the expression of AGK was positively correlated with cell cycle and cell-cycle-dependent protein kinase based on the published gene expression profiles of patients with EOC (the Cancer Genome Atlas TCGA, https://cancergenome.nih.gov/, GSE102180). All experiments were repeated at least three times independently